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1. Topoisomerase I then ligates the PCR product in the correct orientation.
然后拓扑异构酶I将PCR产物按照正确的方向连接。
ligates
2. DCs express TIM4 that ligates TIM1 to drive the proliferation and expansion of Th2 cells.
TIM4主要表达于成熟的树突状细胞，巨噬细胞的膜表面，TIM4与其受体TIM1的结合后，促进Th2细胞的分化与增殖。
3. Intron sequences are removed by RNA splicing that cleaves the RNA at exon-intron boundaries and ligates the ends of the exon sequences together.
RAN 剪接内含子序列由RNA剪接去除，在外显子-内含子的交界处切开RNA，并将外显子的末端连接起来。
4. Some key processes influencing construction of cotton bacterial artificial chromosome library, such as preparation of plug, DNA partial digestion, selection of digested fragment, molecular ratio between insert DNA andvector, concentration of ligates etc, were explored.
以我国抗病、优质、丰产的棉花优良品种中棉所12号为材料，对棉花细菌人工染色体文库构建过程中的一些关键技术，如plug的制备、DNA的部分消化、酶切片段的选择、插入片段与载体的摩尔比值、连接产物的浓缩等进行了研究，建立了构建棉花BAC文库的适宜方法体系。
5. Suspenses and ligates the rectal mucus in several sites of the upper part of hemorrhoid; then makes an arc incision on the cutis distal to anal margin, and under the incision connective tissue and varicosity were stripped off., then the incision was sutured end to end.
采用上端痔上直肠粘膜多点结扎悬吊，下端远离肛缘弧形切开，潜行剥离结缔组织及曲张的静脉血管团，端端对接缝合整复外科肛管的方法。